Establishing Ex Situ Conservation Methods for Dactylorhiza ebudensis and D. traunsteinerioides, a Combination of In Situ Turf Removal and In Vitro Germinations

Authors

  • Berta Millas Xanco Royal Botanic Garden Edinburgh
  • Jaime V. Aguilar
  • Gregory J. Kenicer Royal Botanic Garden Edinburgh
  • Heather McHaffie Royal Botanic Garden Edinburgh

DOI:

https://doi.org/10.24823/Sibbaldia.2012.68

Abstract

Orchidaceae is one of the most diverse flowering plant families in the world, occupying a diverse range of habitats from epiphytes to terrestrial forms. It is also one of the most vulnerable to changes in land use because of its complex ecological requirements. In nature, orchid seed will only grow if infected with a compatible fungus which provides all the carbohydrates and nutrients needed for its development. This mycotrophic mode of nourishment can persist underground for years in some orchids, which makes them difficult to observe in the wild. Understanding their behaviour is essential for their successful propagation and conservation. In an investigation looking into conservation and propagation, turves were lifted from wild populations of two rare Scottish orchid species in order to ensure the best possible association between these species and their growing environment. A combined in vitro experiment was set up for the wild harvested seeds under different media to compare their effects. Two different successful ex situ conservation methods for Dactylorhiza ebudensis and D. traunsteinerioides are presented.

Author Biographies

Berta Millas Xanco, Royal Botanic Garden Edinburgh

Horticulture Student

Gregory J. Kenicer, Royal Botanic Garden Edinburgh

Head of Education

Heather McHaffie, Royal Botanic Garden Edinburgh

Conservation Officer

References

Bidartondo, M.I. & Read, D.J. (2008). Fungal specificity bottlenecks during orchid germination and development. Molecular Ecology 17, 3,707–3,716.

Bonnardeaux, Y., Brundrett, M., Batty, A., Dixon, K., Koch, J. & Sivasithamparam, K. (2007). Diversity of mycorrhizal fungi of terrestrial orchids: compatibility webs, brief encounters, lasting relationships and alien invasions. Mycological Research, 11(1), 51–61.

Brundrett, M.C. (2007). Role of symbiotic relationships in Australian terrestrial orchid conservation. Australian Plant Conservation, 15, 2–7.

Burney, D. (2009). Are we naïve to think we can save rare plants from extinction? Sibbaldia, 7, 61–76. Available online: https://doi.org/10.23823/Sibbaldia/2009.151

Clements, M.A., Muir, H. & Cribb, P.J. (1986). A preliminary report on the symbiotic germination of European terrestrial orchids. Kew Bulletin, 41, 437–445.

Cribb, P.J., Kell, S.P., Dixon, K.W. & Barrett, R.L. (2003). Orchid conservation: a global perspective. In: Dixon, K.W., Kell, S.P., Barrett, R.L. & Cribb, P.J. (eds). Orchid Conservation. Natural History Publications, 1–24. Kota Kinabalu, Sabah.

Curt is, J.T. (1939). The relation of specificity of orchid mycorrhizal fungi to the problem of symbiosis. American Journal of Botany, 26, 390–399.

Harrap, A. & Harrap, S. (2009). Orchids of Britain & Ireland. A Field and Site Guide, 2nd edn. A & C Black Publishers Ltd, London.

Mckendrick, S.L., Leake, J.R., Taylor, D.L. & Read, D.J. (2000). Symbiotic germination and development of myco-heterotrophic plants in nature: ontogeny of Corallorhiza trifida and characterization of its mycorrhizal fungi. New Phytologist, 145, 523–537.

Mitchell, R.B. (1989). Growing hardy orchids from seeds at Kew. The Plantsman, 2, 152–169.

Mitchell, R.B. (1993). Dactylorhizas from seed. Bulletin of the Alpine Garden Society, 61(3), 324–330.

Muir, H.J. (1989). Germination and mycorrhizal fungus compatibility in European orchids. In: Pritchard, H.W. (ed.). Modern Methods in Orchid Conservation: The Role of Physiology, Ecology and Management. Cambridge University Press, Cambridge.

Rae, D. (ed.) (2006). Collection Policy for the Living Collection. Royal Botanic Garden Edinburgh.

Rasmussen, H.N. (1995). Terrestrial Orchids: From Seed to Mycotrophic Plant. Cambridge University Press, Cambridge.

Rasmussen, H.N. & Whigham, D.F. (1993). Seed ecology of dust seeds in situ: A new study technique and its application in terrestrial orchids. American Journal of Botany, 80(12), 1,374–1,378.

Seaton , P. & Ramsey, M. (2005). Growing Orchids from Seed. Royal Botanic Gardens, Kew. Kewgrowing, Surrey.

Seaton , P., Cribb, P., Ramsey, M. & Hagger, J. (2012). Growing Hardy Orchids. Royal Botanic Gardens, Kew. Kewgrowing, Surrey.

Single, J. & Single, S. (2010). Short Note: Good roots matter from day one. Sibbaldia, 8, 179–188. Available online: https://doi.org/10.23823/Sibbaldia/2010.145

Swarts , N.D. & Dixon, K.W. (2009). Terrestrial orchid conservation in the age of extinction. Annals of Botany, 104, 543–556.

Swarts , N.D., Sinclair, E.A., Francis, A. & Dixon, K.W. (2010). Ecological specialization in mycorrhizal symbiosis leads to rarity in an endangered orchid. Molecular Ecology, 19, 3,226–3,242.

Thompson, P.A. (1977). Orchids from Seed. HMSO, London.

Vermeulen, P. (1947). Studies on Dactylorchids. Schotanus & Jens, Utrecht.

Walter, K.S. & O’Neal, M.J. (1985–2010). BG-BASETM (Collection Management Software). BG-BA SE Inc. & BG-BA SE (UK) Ltd.

Waterman, R.J. & Bidartondo, M.I. (2007). Deception above, deception below: linking pollination and mycorrhizal biology of orchids. Journal of Experimental Botany, 59(5), 1,085–1,096.

Yoder, J.A., Zettler, L.W. & Stewart , S.L. (2000). Water requirements of terrestrial and epiphytic orchid seeds and seedlings, and evidence for water uptake by means of mycotrophy. Plant Science, 156, 145–150.

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Published

2012-10-31

How to Cite

Xanco, B. M., Aguilar, J. V., Kenicer, G. J., & McHaffie, H. (2012). Establishing Ex Situ Conservation Methods for Dactylorhiza ebudensis and D. traunsteinerioides, a Combination of In Situ Turf Removal and In Vitro Germinations. Sibbaldia: The International Journal of Botanic Garden Horticulture, (10), 71–84. https://doi.org/10.24823/Sibbaldia.2012.68

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