Preliminary Observations on Fruit Handling, Seed Germination and Chloroplast Inheritance of an Amenotaxus Hybrid Arising at the Royal Botanic Garden Edinburgh from A. argotaenia (F) x A. formosana (M)

Authors

  • Peter G Gosling Forestry Commission Research Agency
  • Shelagh A McCartan Forestry Commission Research Agency
  • Lorelie M Ives Forestry Commission Research Agency
  • Victoria J. Cunningham Forestry Commission Research Agency
  • Jane Squirrell Royal Botanic Garden Edinburgh
  • Philip Thomas Royal Botanic Garden Edinburgh

DOI:

https://doi.org/10.24823/Sibbaldia.2008.37

Abstract

Seed quality of Amentotaxus species is generally considered to be poor. This paper describes some general observations on the collection, processing and germination of seeds of an Amentotaxus hybrid (A. argotaenia [F] 3x A. formosana [M]) that originated at the Royal Botanic Garden Edinburgh in 2005. It also includes the details of five different methods for incubating/culturing the hybrid seeds – three of these methods resulted in almost 100% germination. DNA analysis confirmed the hybrid origin of the seeds and in common with many conifers, chloroplast inheritance was shown to be via the paternal side.

Author Biographies

  • Peter G Gosling, Forestry Commission Research Agency
    Peter G. Gosling is Project Leader
  • Shelagh A McCartan, Forestry Commission Research Agency
    Shelagh A. McCartan is a Project Support Officer
  • Lorelie M Ives, Forestry Commission Research Agency
    Lorelie M. Ives is a Project Support Officer
  • Victoria J. Cunningham, Forestry Commission Research Agency
    Victoria J. Cunningham is a Project Support Officer
  • Jane Squirrell, Royal Botanic Garden Edinburgh
    Jane Squirrell is a Molecular Ecologist and Conservation Geneticist
  • Philip Thomas, Royal Botanic Garden Edinburgh
    Philip Thomas is Scientific Officer on the International Conifer Conservation Programme

References

CHEN, Z.-K. & WANG, F.-X. (1984). Early embryogeny and its starch distribution in Amentotaxus. Acta Botanica Sinica, 26(4), 359–364.

FARJON, A. (2001). World Checklist and Bibliography of Conifers. Second edition. RBG Kew, Surrey. 309 pp.

FERGUSON, D.K. (1992). A Krausel legacy: Advances in our knowledge of the taxonomy and evolution in Amentotaxus. Cour. Forsch. – Inst. Senckenberg, 147, 255–285. In: International Symposium on Palaeobotany “Anatomical Investigations of Plant Fossils”. Ed. Friedemann Schaarschmidt.

GADEK, P.A., ALPERS, D.L., HESLEWOOD, M.M. & QUINN, C.J. (2000). Relationships within Cupressaceae sensu lato: a combined morphological and molecular approach. Amer. J. Bot., 87, 1044–1057.

GE, X.J., ZHOU, X.L., LI, Z.C., HSU, T.W., SCHAAL, B.A. & CHIANG, T.Y. (2005). Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting. J. Plant Res., 118, 415–422.

GOSLING, P.G., IVES, L., CUNNINGHAM, V., HECHENLEITNER-VEGA, P., BROWNLESS, P., THOMAS, P. & MARTINEZ, C. (2005). Preliminary advice on fruit handling, seed pretreatment and ‘germination’ of embryos of Prumnopitys andina. Sibbaldia, 3, 41–50.

HIEP, N.T. & VIDAL , J.E. (1996). Flore du Cambodge, du Laos et du Vietnam. Vol. 28. Gymnospermae. Muséum National d’Histoire Naturelle, Paris.

HIEP, N.T., LOC, P.K., LUU, N.D.T., THOMAS, P.I., FARJON, A., AVERYANOV, L. & REGALADO, J. Jr. (2004). Vietnam Conifers: Conservation Status Review 2004. Fauna & Flora International, Vietnam Programme, Hanoi.

ISTA (2005). International Rules for Seed Testing. Publ. International Seed Testing Association, Basserdorf, Switzerland.

IUCN (2007). Amentotaxus: (2007) IUCN Red List of Threatened Species. Available online: http://www.iucnredlist.org. (Accessed 13 September 2007)

LUU, N.D.T. & THOMAS, P. (2004). Cay La Kim Vietnam / Conifers of Vietnam: an illustrated field guide. World Publishing House, Hanoi. 121 pp. (in Vietnamese and English). Darwin Initiative. ISBN 1 872291 64 3. Available online : http://www.ceh.ac.uk/sections/bm/conifer_manual.html (Accessed 3rd July 2006).

PAN, F. & YANG, J. (1996). An _ex situ_ conservation plan for endangered species of Taiwan. Pp. 108–117 in HUNT, D. (ed.). Temperate trees under threat. Proceedings of an IDS symposium on the conservation status of temperate trees. University of Bonn, 30 September–1 October 1994. Great Britain: IDS. 200 pp.

RUSHFORTH, K. (1987). Conifers. Facts on File Publications, New York and Oxford. 232 pp.

TABERLET, P., GIELLY, L., PAUTOU, G. & BOUVET, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17, 1105–1109.

THOMAS, P., SENGDALA , K., LAMXAY, V. & KHOU, E. (2007). New records of conifers in Cambodia and Laos. Edinburgh Journal of Botany, 64(1), 37–44.

WANG, D., LI, Z. & GE, X. (2005). Genetic diversity of the relict plant Amentotaxus yunnanensis. Journal of Tropical and Subtropical Botany, 13(2), 143–148.

Downloads

Published

2008-10-31

Issue

Section

Articles

How to Cite

Preliminary Observations on Fruit Handling, Seed Germination and Chloroplast Inheritance of an Amenotaxus Hybrid Arising at the Royal Botanic Garden Edinburgh from A. argotaenia (F) x A. formosana (M). (2008). Sibbaldia: The International Journal of Botanic Garden Horticulture, 6, 101-114. https://doi.org/10.24823/Sibbaldia.2008.37

Most read articles by the same author(s)