HOW UNIVERSAL ARE UNIVERSAL rDNA PRIMERS? A CAUTIONARY NOTE FOR PLANT SYSTEMATISTS AND PHYLOGENETICISTS

Authors

  • M. MÖLLER Royal Botanic Garden Edinburgh

DOI:

https://doi.org/10.1017/S096042860000010X

Keywords:

Internal transcribed spacer, nucleolar organizer regions, PCR, ribosomal DNA, universal primers

Abstract

Phylogenetic relationships are frequently inferred from rDNA ITS sequences obtained employing universal primers. Whereas in most cases phylogenetic topologies inferred from ITS data make good biological sense, caution has to be used when a number of taxa vary greatly in PCR yield. The phylogenetic implications of such a scenario are discussed.

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Published

2000-12-04

Issue

Vol. 57 No. 2 (2000)

Section

Original Research Articles

License

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